In Situ Imaging and Anti-inflammation of 3D Printed Scaffolds Enabled by AIEgen.
Xiaoxuan WangPu ChenHe YangJiawei LiuRong TuHai-Tao FengHongLian DaiPublished in: ACS applied materials & interfaces (2023)
Three-dimensional (3D) printed bioactive scaffolds have been widely used in the field of bone tissue engineering. However, its in vivo visualization and bacterial inflammation are intractable issues during the surgery and treatment. Herein, we first synthesized an aggregation-induced emission-active luminogen (AIEgen) named 4BC with efficient reactive oxygen species (ROS) generation. Then, a series of 3D bioactive scaffolds loaded with 4BC were fabricated by a precipitation adsorption method, namely 4BC @scaffolds, which showed good in situ imaging performance for the implanted scaffolds by using simple UV light irradiation. Among them, the 4BC @TMP scaffold composed of trimagnesium phosphate (TMP) had excellent bactericidal ability for Escherichia coli and Staphylococcus aureus in vitro and resisted bacterial inflammation in vivo through photodynamic action. H&E and immunofluorescence staining were performed to further evaluate the inhibitory effect of bacterial inflammation in vivo. This work verified that AIEgen-based 3D scaffolds are promising bioactive frameworks for bioimaging and antibacterial applications.
Keyphrases
- tissue engineering
- oxidative stress
- reactive oxygen species
- escherichia coli
- staphylococcus aureus
- high resolution
- minimally invasive
- dna damage
- cell death
- cystic fibrosis
- combination therapy
- pseudomonas aeruginosa
- living cells
- radiation induced
- multidrug resistant
- fluorescence imaging
- bone mineral density
- coronary artery disease
- wound healing
- methicillin resistant staphylococcus aureus
- percutaneous coronary intervention