Deoxyglucose-conjugated persistent luminescent nanoparticles for theragnostic application in fibrosarcoma tumor model.
K S SharmaPooja K MelwaniHansa D YadavRashmi JoshiNeena G ShetakeAkhil K DubeyBheeshma Pratap SinghSuhas PhapalePrasad P PhadnisRajesh K VatsaRaghumani Singh NingthoujamBadri N PandeyPublished in: RSC advances (2023)
Deoxyglucose conjugated nanoparticles with persistent luminescence have shown theragnostic potential. In this study, deoxyglucose-conjugated nano-particles with persistent luminescence properties were synthesized, and their theragnostic potential was evaluated in fibrosarcoma cancer cells and a tumor model. The uptake of nano-formulation was found to be higher in mouse fibrosarcoma (WEHI-164) cells cultured in a medium without glucose. Nanoparticles showed a higher killing ability for cancer cells compared to normal cells. A significant accumulation of nanoparticles to the tumor site in mice was evident by the increased tumor/normal leg ratio, resulting in a significant decrease in tumor volume and weight. Histopathological studies showed a significant decrease in the number of dividing mitotic cells but a greater number of apoptotic/necrotic cells in nanoparticle-treated tumor tissues, which was correlated with a lower magnitude of Ki-67 expression (a proliferation marker). Consequently, our results showed the potential of our nano-formulation for cancer theragnosis.
Keyphrases
- induced apoptosis
- cell cycle arrest
- endoplasmic reticulum stress
- signaling pathway
- photodynamic therapy
- quantum dots
- cell death
- body mass index
- oxidative stress
- gene expression
- endothelial cells
- squamous cell carcinoma
- metabolic syndrome
- risk assessment
- cell proliferation
- human health
- skeletal muscle
- weight gain
- physical activity
- radiation therapy
- blood glucose