TLC-ESI-MS as a QuEChERS approach to detection of capsaicin present in different matrices.
Filipe Kayodè Felisberto Dos SantosLeandro da Silva NascimentoSamir Frontino de Almeida CavalcanteClaudia Moraes RezendeValdir Florêncio da Veiga-JuniorPublished in: Natural product research (2021)
Pepper is one of the most consumed spices in the world. Its main compound is capsaicin, a widely studied biomarker that has pharmacological activities due to its anti-inflammatory and analgesic capacity. Topical formulations such as patches based on capsaicin have been developed as an option in relieving pain and reducing swelling. In addition, capsaicin is used as an active ingredient in non-lethal weapon formulations such as pepper spray through the QuEChERS concept (Quick, Easy, Cheap, Effective, Robust, Safe) technique. Used for food analysis, it allows the direct analysis of the biomarker by TLC-ESI-MS, which are previously separated by HPTLC (High Performance Thin Layer Chromatography) using an internal standard for determination of Rf and confirmation of capsaicin in different matrices.
Keyphrases
- ms ms
- mass spectrometry
- anti inflammatory
- liquid chromatography tandem mass spectrometry
- tandem mass spectrometry
- high performance liquid chromatography
- neuropathic pain
- multiple sclerosis
- ultra high performance liquid chromatography
- gas chromatography
- liquid chromatography
- chronic pain
- high resolution mass spectrometry
- simultaneous determination
- spinal cord
- high resolution
- gas chromatography mass spectrometry
- molecularly imprinted
- climate change
- solid state
- postoperative pain