Osseointegration mechanisms: a proteomic approach.
N Araújo-GomesFrancisco J Romero-GavilánI García-ArnáezC Martínez-RamosA M Sánchez-PérezM AzkargortaF ElortzaJ J Martín de LlanoM GurruchagaI GoñiJ SuayPublished in: Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry (2018)
The prime objectives in the development of biomaterials for dental applications are to improve the quality of osseointegration and to short the time needed to achieve it. Design of implants nowadays involves changes in the surface characteristics to obtain a good cellular response. Incorporating osteoinductive elements is one way to achieve the best regeneration possible post-implantation. This study examined the osteointegrative potential of two distinct biomaterials: sandblasted acid-etched titanium and a silica sol-gel hybrid coating, 70% MTMOS-30% TEOS. In vitro, in vivo, and proteomic characterisations of the two materials were conducted. Enhanced expression levels of ALP and IL-6 in the MC3T3-E1 cells cultured with coated discs, suggest that growing cells on such surfaces may increase mineralisation levels. 70M30T-coated implants showed improved bone growth in vivo compared to uncoated titanium. Complete osseointegration was achieved on both. However, coated implants displayed osteoinductive properties, while uncoated implants demonstrated osteoconductive characteristics. Coagulation-related proteins attached predominantly to SAE-Ti surface. Surface properties of the material might drive the regenerative process of the affected tissue. Analysis of the proteins on the coated dental implant showed that few proteins specifically attached to its surface, possibly indicating that its osteoinductive properties depend on the silicon delivery from the implant.
Keyphrases
- soft tissue
- induced apoptosis
- stem cells
- cell cycle arrest
- tissue engineering
- mesenchymal stem cells
- oral health
- bone regeneration
- endoplasmic reticulum stress
- endothelial cells
- escherichia coli
- cell death
- risk assessment
- cell therapy
- bone mineral density
- staphylococcus aureus
- quality improvement
- cystic fibrosis
- african american
- climate change
- human health
- bone loss