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Evaluation of diagnostic methods for the detection of intestinal schistosomiasis in endemic areas with low parasite loads: Saline gradient, Helmintex, Kato-Katz and rapid urine test.

Warllem Junio OliveiraFernanda do Carmo MagalhãesAndressa Mariana Saldanha EliasVanessa Normandio de CastroVivian FaveroCatieli Gobetti LindholzÁureo Almeida OliveiraFernando Sergio BarbosaFrederico GilMaria Aparecida GomesCarlos Graeff-TeixeiraMartin Johannes EnkPaulo Marcos Zech CoelhoMariângela CarneiroDeborah Aparecida Negrão-CorrêaStefan Michael Geiger
Published in: PLoS neglected tropical diseases (2018)
The recommended Kato-Katz method largely underestimated the prevalence of S. mansoni infection. Because the best performance was achieved with a modified Helmintex method, this technique might serve as a more precise reference standard. An extended number of Kato-Katz slides in combination with other parasitological methods or with POC-CCA was able to detect more than 80% of egg-positive individuals; however, the rapid urine test (POC-CCA) produced a considerable percentage of false positive results.
Keyphrases
  • loop mediated isothermal amplification
  • risk factors
  • sensitive detection
  • real time pcr
  • label free
  • plasmodium falciparum
  • life cycle
  • trypanosoma cruzi