Ag nanoparticle in situ decorated on Ti 3 C 2 T x with excellent SERS and EIS immunoassay performance for beta-human chorionic gonadotropin.

Two-dimensional transition metal carbides, nitrides, and carbonitrides (MXene), with excellent optical and electrical properties, are promising substrates for surface-enhanced Raman scattering (SERS) and electrochemical sensors. Therefore, a unique 3D-decorated structure containing silver (Ag) nanoparticles and Ti 3 C 2 T x was designed as the substrates of SERS and electrochemical impedance spectroscopy (EIS) immunosensors. The Ag/Ti 3 C 2 T x composite significantly increases Raman intensity, which is attributed to the synergistic effect of Ti 3 C 2 T x and Ag nanoparticles. Based on the SERS performance of the Ag/Ti 3 C 2 T x composite, the magnetic properties of Fe 3 O 4 and the specificity of antigen-antibody, a sandwich-structured SERS immunosensor is constructed, which can effectively detect trace amounts of beta-human chorionic gonadotropin (β-hCG). The SERS immunosensor exhibits a wide linear range of 5.0 × 10 -6 -1.0 mIU mL -1 , and a low detection limit of 9.0 × 10 -7 mIU mL -1 . Meanwhile, the Ag/Ti 3 C 2 T x -based EIS immunosensor is constructed for the portable detection of β-hCG, which exhibits a wide linear range of 5.0 × 10 -2 -1.0 × 10 2 mIU mL -1 , a low detection limit of 9.5 × 10 -3 mIU mL -1 . Moreover, two immunosensors can be used to detect actual serum samples with satisfactory recovery (98.5-102.2%). This work could guide the design of low-cost, sensitive, flexible, and portable biosensors. The SERS and EIS substrates composited with Ti 3 C 2 T x and Ag nanoparticles enable excellent performance for detecting β-hCG.