Anti-inflammatory and anti-arthritic effects of compounds from Buddleja coriacea .

Buddleja coriacea ( B. coriacea ) commonly known as 'Kiswara' is used as infusions for stomach and joint pain. This study aims to evaluate the in vitro and in vivo anti-inflammatory and anti-arthritic activity was evaluated by measuring inflammatory parameters (TNF- α , C-reactive protein and Fibrinogen) in murine models of the aqueous extract and isolated compounds of B. coriacea . A bio-guided phytochemical analysis based on NMR/MS was performed identifying three ( 1 - 3 ) compounds of the aqueous extract. All compounds inhibited the production of TNF- α in RAW 264.7 cell line, with IC 50 of 13.44 ( 1 ), 1.13 ( 2 ) and 0.57 μM ( 3 ), respectively. In addition, compounds 2 and 3 decreased the levels of TNF- α , C-reactive protein and fibrinogen at a concentration of 5 mg/kg in murine models. Our research shows that the compounds isolated from B. coriacea have anti-inflammatory and anti-arthritic properties, providing scientific evidence for the traditional use of this plant species.