Isolation and characterization of a SARS-CoV-2 variant with a Q677H mutation in the spike protein.
Xiaoyu NiuJiayu XuMingde LiuHuolin TuSara N KoenigLinda J SaifDaniel M JonesQiuhong WangPublished in: Archives of virology (2022)
We isolated 20 SARS-CoV-2 strains from positive clinical samples collected in Columbus, Ohio, and investigated the replication of one pair of isolates: a clade 20G strain and a variant of this strain carrying a Q677H mutation in the spike protein and six other amino acid mutations. The OSU.20G variant replicated to a higher peak infectious titer than the 20G base strain in Vero-E6 cells, but the titers were similar when both strains were grown in Calu-3 cells. These results suggest that the OSU.20G variant has increased replication fitness compared to the 20G base strain. This may have contributed to its emergence in December 2020-January 2021.
Keyphrases
- sars cov
- induced apoptosis
- amino acid
- cell cycle arrest
- escherichia coli
- respiratory syndrome coronavirus
- physical activity
- endoplasmic reticulum stress
- cell death
- oxidative stress
- binding protein
- signaling pathway
- body composition
- small molecule
- cell proliferation
- coronavirus disease
- pi k akt
- mass spectrometry
- single molecule