Hydrophilic Carbon Nanomaterials: Characterisation by Physical, Chemical, and Biological Assays.
Andreia D VelosoAna M FerrariaAna M Botelho do RegoPedro B TavaresPatrícia ValentãoDavid D PereiraPaula B AndradeAntónio J FernandesMaria C OliveiraRomeu António VideiraPublished in: ChemMedChem (2019)
A highly hydrophilic carbon nanomaterial was generated by using an electrochemical approach, and its structure, chemical composition, redox properties, antioxidant activity and effects on cells were characterised. It was found that the nanomaterial possesses a structure dominated by sp2 carbon atoms in a non-ordered carbon network formed by small clusters (<2 nm) of a carbonaceous material. This material has an outstanding capability for donating electrons and an unusual ability to bind metal cations. Antioxidant activity assays showed that it displays a high scavenging activity against both 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radicals, and a concentration-dependent ability to protect mitochondrial lipids and intracellular thiol groups from oxidation promoted by external oxidising agents. Cell-based assays also revealed that the nanomaterial has the ability to protect neuronal cells against oxidative damage and toxicity promoted by tert-butyl hydroperoxide and amyloid-β1-42 peptide. These results, combined with the attractive methodology for generating this hydrophilic carbon-based nanomaterial, make this study the first step in addressing the therapeutic application of this new material.
Keyphrases
- induced apoptosis
- liquid chromatography
- high throughput
- oxidative stress
- cell cycle arrest
- ionic liquid
- single cell
- mental health
- physical activity
- mass spectrometry
- endoplasmic reticulum stress
- mesenchymal stem cells
- cell death
- nitric oxide
- bone marrow
- blood brain barrier
- molecularly imprinted
- subarachnoid hemorrhage
- brain injury