A single-step, rapid and versatile method for simultaneous detection of cell surface glycan profiles using fluorochrome-conjugated lectins.
Nicolás I TorresMontana N Manselle CoccoRamiro M PerrotaYamil D MahmoudMariana SalatinoKarina V MariñoGabriel A RabinovichPublished in: Glycobiology (2023)
Cell surface glycans play essential roles in diverse physiological and pathological processes and their assessment has important implications in biomedicine and biotechnology. Here we present a rapid, versatile and single-step multicolor flow cytometry method for evaluation of cell surface glycan signatures using a panel of selected fluorochrome-conjugated lectins. This procedure allows simultaneous detection of cell surface glycans with a 10-fold reduction in the number of cells required compared to traditional multistep lectin staining methods. Interestingly, we used this one-step lectin array coupled with dimension reduction algorithms in a proof-of-concept application for discrimination among different tumor and immune cell populations. Moreover, this procedure was also able to unveil T, B and myeloid cell sub-clusters exhibiting differential glycophenotypes. Thus, we report a rapid and versatile lectin cytometry method to simultaneously detect a particular repertoire of surface glycans on living cells that can be easily implemented in different laboratories and core facilities.
Keyphrases
- cell surface
- loop mediated isothermal amplification
- flow cytometry
- living cells
- sensitive detection
- single cell
- fluorescent probe
- induced apoptosis
- photodynamic therapy
- machine learning
- minimally invasive
- single molecule
- acute myeloid leukemia
- real time pcr
- label free
- stem cells
- dendritic cells
- cell cycle arrest
- cell therapy
- high throughput
- bone marrow
- gene expression
- cell death
- oxidative stress
- mass spectrometry
- endoplasmic reticulum stress
- high density
- genetic diversity
- quantum dots