Mapping the conformational epitope of a therapeutic monoclonal antibody against HBsAg by in vivo selection of HBV escape variants.
Chang-Ru WuHyun-Jin KimCheng-Pu SunChen-Yen ChungYou-Yu LinMi-Hua TaoJung-Hwan KimDing-Shinn ChenPei-Jer ChenPublished in: Hepatology (Baltimore, Md.) (2022)
Selection of antibody-escape HBV variants in human chimeric mice works efficiently. Analysis of such emerging variants helps to identify anchor amino-acid residues of the conformational epitope that are difficult to discover by conventional approaches.
Keyphrases
- monoclonal antibody
- hepatitis b virus
- copy number
- amino acid
- molecular dynamics
- molecular dynamics simulations
- endothelial cells
- single molecule
- liver failure
- cell therapy
- dna methylation
- high fat diet induced
- induced pluripotent stem cells
- genome wide
- gene expression
- bone marrow
- mass spectrometry
- insulin resistance
- mesenchymal stem cells