Highly sensitive turn-off fluorescent detection of cyanide in aqueous medium using dicyanovinyl-substituted phenanthridine fluorophore.
Saravanakumar ManickamSathiyanarayanan Kulathu IyerPublished in: RSC advances (2020)
Herein, the turn-off fluorescence sensor of 2-((4'-(7,8,13,14-tetrahydrodibenzo[ a , i ]phenanthridin-5-yl)-[1,1'-biphenyl]-4-yl)methylene)malanonitrile (7) was developed for the recognition of CN - ions and studied using different spectroscopic techniques. The selective recognition of CN - ions by 7 was investigated via UV-vis spectroscopy and fluorescence studies in acetonitrile solvent, which exhibited an obvious color change from orange to colorless under 365 UV light. The sensor compound 7 possesses a high binding constant ( K a ) for CN - ions in the order of 5.22 × 10 6 M -1 . The results from the interference studies revealed that probe 7 shows high sensing selectivity and sensitivity for CN - ions over other competitive anions. Probe 7 interacts with cyanide to form a 1 : 1 adduct, and this mechanism was further verified by 1 H NMR titration, Job's plot analyses and DFT studies. The sensor probe 7 exhibits advantages such as low limit of detection (LOD) of 39.3 nM, fast response and sensing in a wide pH range of 3 to 11. The practical application of 7 was successfully demonstrated for the determination of CN - ions in test paper strips and various water samples.
Keyphrases
- living cells
- fluorescent probe
- quantum dots
- lymph node metastasis
- sensitive detection
- aqueous solution
- single molecule
- energy transfer
- molecular docking
- loop mediated isothermal amplification
- ionic liquid
- label free
- case control
- high resolution
- solid state
- water soluble
- real time pcr
- depressive symptoms
- single cell
- molecular dynamics