Evaluation of Blood-CSF Barrier Transport by Quantitative Real Time Fluorescence Microscopy.

Published in: Pharmaceutical research (2022)

A CP isolation method was described, and a quantitative fluorescence imaging approach was developed to evaluate CSF-to-blood transport in mouse CP. The method is consistent, reproducible, and capable of tracking real time transepithelial transport with temporal and spatial resolution. The approach can be used to evaluate transport mechanisms, assess tissue drug accumulation, and assay potential drug-drug interactions at the BCSFB.

Keyphrases

fluorescence imaging
single molecule
high resolution
high throughput
photodynamic therapy
optical coherence tomography
adverse drug
high speed
climate change
cerebrospinal fluid
single cell
human health