3D Chemical Imaging by Fluorescence-detected Mid-Infrared Photothermal Fourier Light Field Microscopy.
Danchen JiaYi ZhangQianwan YangYujia XueYuying TanZhongyue GuoMeng ZhangLei TianJi-Xin ChengPublished in: Chemical & biomedical imaging (2023)
Three-dimensional molecular imaging of living organisms and cells plays a significant role in modern biology. Yet, current volumetric imaging modalities are largely fluorescence-based and thus lack chemical content information. Mid-infrared photothermal microscopy as a chemical imaging technology provides infrared spectroscopic information at submicrometer spatial resolution. Here, by harnessing thermosensitive fluorescent dyes to sense the mid-infrared photothermal effect, we demonstrate 3D fluorescence-detected mid-infrared photothermal Fourier light field (FMIP-FLF) microscopy at the speed of 8 volumes per second and submicron spatial resolution. Protein contents in bacteria and lipid droplets in living pancreatic cancer cells are visualized. Altered lipid metabolism in drug-resistant pancreatic cancer cells is observed with the FMIP-FLF microscope.
Keyphrases
- single molecule
- high resolution
- drug resistant
- photodynamic therapy
- cancer therapy
- living cells
- drug delivery
- drug release
- multidrug resistant
- optical coherence tomography
- high throughput
- fluorescence imaging
- label free
- healthcare
- induced apoptosis
- mass spectrometry
- energy transfer
- machine learning
- big data
- molecular docking
- signaling pathway
- cystic fibrosis
- amino acid
- cell proliferation
- artificial intelligence
- single cell
- molecular dynamics simulations