A Simple Near-Infrared Fluorescent Probe for the Detection of Peroxynitrite.

Luling Wu Xue Tian Hai-Hao Han Jie Wang Robin R GroleauParamabhorn TosuwanBoontana WannalerseAdam C Sedgwick Steven D Bull Xiao-Peng He Tony David James

Published in: ChemistryOpen (2019)

Herein, we report the evaluation and synthesis of a reaction based fluorescent probe DCM-Bpin for the detection of Peroxynitrite (ONOO-). DCM-Bpin exhibits selective fluorescence off-on response for ONOO- over other reactive oxygen species, including H2O2. Moreover, DCM-Bpin is biocompatible and has been used to visualize exogenous ONOO- in HeLa cells.

Keyphrases

fluorescent probe
living cells
reactive oxygen species
cell cycle arrest
loop mediated isothermal amplification
induced apoptosis
label free
real time pcr
cell death
oxidative stress
signaling pathway
sensitive detection
energy transfer