let-7 coordinates the transition to adulthood through a single primary and four secondary targets.
Florian AeschimannAnca NeaguMagdalene RauschHelge GroßhansPublished in: Life science alliance (2019)
The juvenile-to-adult (J/A) transition, or puberty, is a period of extensive changes of animal body morphology and function. The onset of puberty is genetically controlled, and the let-7 miRNA temporally regulates J/A transition events in nematodes and mammals. Here, we uncover the targets and downstream pathways through which Caenorhabditis elegans let-7 controls male and female sexual organ morphogenesis and skin progenitor cell fates. We find that let-7 directs all three processes by silencing a single target, the post-transcriptional regulator lin-41 In turn, the RNA-binding protein LIN41/TRIM71 regulates these processes by silencing only four target mRNAs. Thus, by silencing LIN41, let-7 activates LIN-29a and MAB-10 (an early growth response-type transcription factor and its NAB1/2-orthologous cofactor, respectively) to terminate progenitor cell self-renewal and to promote vulval integrity. By contrast, let-7 promotes development of the male sexual organ by up-regulating DMD-3 and MAB-3, two Doublesex/MAB-3 domain-containing transcription factors. Our results provide mechanistic insight into how a linear chain of post-transcriptional regulators diverges in the control of a small set of transcriptional regulators to achieve a coordinated J/A transition.
Keyphrases
- transcription factor
- dna binding
- binding protein
- monoclonal antibody
- genome wide identification
- mental health
- magnetic resonance
- gene expression
- depressive symptoms
- magnetic resonance imaging
- soft tissue
- sensitive detection
- computed tomography
- early life
- oxidative stress
- fluorescent probe
- advanced non small cell lung cancer
- wound healing