Ultra-sensitive electrochemical immunosensor based on 2D vanadium diselenide (VSe 2 ) for efficient detection of pathogens: Salmonella Typhimurium.
Rim M AlsharabiJay SinghPreeti S SaxenaAnchal SrivastavaPublished in: Luminescence : the journal of biological and chemical luminescence (2024)
Layered transition metal dichalcogenides (TMDs), with an extensive surface area, intriguing tunable electrical and optical features, and a distinctive Van der Waals layered structure, yield outstanding sensing properties. Essentially, most TMDs originally existed in the crystallographic phase of a 2H trigonal prismatic structure, which is semiconducting in nature with poor electrocatalytic activity. In contrast, vanadium diselenide (VSe 2 ) with its metastable metallic 1 T octahedral crystal structure has been proven to be an outstanding electrode material, embracing exceptional electrocatalytic behavior for various electrochemical (EC) applications. However, practically, VSe 2 has hardly ever been explored in the field of biosensing technology. This study presents a novel EC biosensor based on the antibody of Salmonella Typhimurium (Anti-ST) immobilized on VSe 2 -supported Indium tin oxide (Anti-ST/VSe 2 /ITO) for quantitative and efficient Salmonella Typhimurium (ST) detection. The Anti-ST/VSe 2 /ITO bioelectrode displayed a linear relationship with ST concentration (1.3 × 10-10 7 CFU/ml) with a limit of detection (LOD) (0.096 CFU/ml) that is lower than previously reported ST biosensors and impressively high sensitivity (0.001996 μA.mL/CFU). Furthermore, the proposed electrode's electroanalytical activity was evaluated in spiked sugarcane juice, demonstrating distinguished applicability for specific ST detection in real samples.
Keyphrases
- label free
- listeria monocytogenes
- transition metal
- escherichia coli
- gold nanoparticles
- crystal structure
- real time pcr
- loop mediated isothermal amplification
- reduced graphene oxide
- high resolution
- magnetic resonance
- computed tomography
- solid state
- antimicrobial resistance
- highly efficient
- mass spectrometry
- multidrug resistant