Modeling of solar UV-induced photodamage on the hair follicles in human skin organoids.
Min-Ji KimHee-Jin AhnDasom KongSeunghee LeeDa-Hyun KimKyung Sun KangPublished in: Journal of tissue engineering (2024)
Solar ultraviolet (sUV) exposure is known to cause skin damage. However, the pathological mechanisms of sUV on hair follicles have not been extensively explored. Here, we established a model of sUV-exposed skin and its appendages using human induced pluripotent stem cell-derived skin organoids with planar morphology containing hair follicles. Our model closely recapitulated several symptoms of photodamage, including skin barrier disruption, extracellular matrix degradation, and inflammatory response. Specifically, sUV induced structural damage and catagenic transition in hair follicles. As a potential therapeutic agent for hair follicles, we applied exosomes isolated from human umbilical cord blood-derived mesenchymal stem cells to sUV-exposed organoids. As a result, exosomes effectively alleviated inflammatory responses by inhibiting NF-κB activation, thereby suppressing structural damage and promoting hair follicle regeneration. Ultimately, our model provided a valuable platform to mimic skin diseases, particularly those involving hair follicles, and to evaluate the efficacy and underlying mechanisms of potential therapeutics.
Keyphrases
- soft tissue
- wound healing
- high glucose
- oxidative stress
- extracellular matrix
- diabetic rats
- cord blood
- inflammatory response
- stem cells
- signaling pathway
- endothelial cells
- mesenchymal stem cells
- induced pluripotent stem cells
- drug induced
- multidrug resistant
- immune response
- risk assessment
- depressive symptoms
- nuclear factor