qPCR assay optimisation for a clinical study comparing oral health risk in Rett syndrome.

Yvonne Yee Lok LaiJ DownsS LeishmanH M LeonardL J WalshS Zafar

Published in: European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry (2024)

Assays were optimised for detection and evaluation of oral health risk in dental plaque samples stored on FTA cards when cold storage is not feasible, except for F. nucleatum. Several assays showed gene copy numbers less than the LOQ or outside the range of the standard curve, so there is merit in optimising these assays using digital droplet PCR.

Keyphrases

high throughput
oral health
health risk
heavy metals
single cell
drinking water
real time pcr
coronary artery disease
copy number
genome wide
room temperature
gene expression
loop mediated isothermal amplification
risk assessment
clinical trial
transcription factor
ionic liquid