Bioinformatic Analysis of the Subproteomic Profile of Cardiomyopathic Tissue.
Sandra MurphyMargit ZweyerDieter SwandullaKay OhlendieckPublished in: Methods in molecular biology (Clifton, N.J.) (2022)
Following large-scale protein separation by two-dimensional gel electrophoresis or liquid chromatography, mass spectrometry-based proteomics can be used for the swift identification and characterization of cardiac proteins and their various proteoforms. Comparative cardiac proteomics has been widely applied for the systematic analysis of heart disease and the establishment of novel diagnostic protein biomarkers. The X-linked neuromuscular disorder Duchenne muscular dystrophy is a multisystemic disease that is characterized by late-onset cardiomyopathy. This chapter outlines the bioinformatic analysis of the subproteomic profile of cardiac tissue from wild-type versus the dystrophic mdx-4cv mouse model of dystrophinopathy.
Keyphrases
- mass spectrometry
- liquid chromatography
- duchenne muscular dystrophy
- late onset
- left ventricular
- high resolution mass spectrometry
- mouse model
- wild type
- tandem mass spectrometry
- early onset
- gas chromatography
- high performance liquid chromatography
- high resolution
- capillary electrophoresis
- heart failure
- binding protein
- muscular dystrophy
- solid phase extraction
- ms ms