Angiotensin-converting enzyme insertion/deletion (rs106180) and angiotensin type 1 receptor A1166 C (rs106165) genotypes and psoriasis: Correlation with cellular immunity, lipid profile, and oxidative stress markers.
Maryam TanhapourBadieh FalahiAsad Vaisi-RayganiFariborz BahrehmandAmir KianiZohreh RahimiAli-Akbar Vaisi-RayganiEbrahim ShakibaTayebeh PourmotabbedPublished in: Journal of cellular biochemistry (2018)
Psoriasis is a chronic inflammatory skin condition and angiotensin-converting enzyme (ACE) is a key circulating enzyme converting angiotensin (Ang) I to the vasoactive peptide Ang II. The exact role of ACE insertion (I)/deletion (D) polymorphism (rs106180) in psoriasis is not clear. We aimed to examine whether the ACE I/D and Ang II type 1 receptor (AT1R) A1166 C-polymorphisms (rs106165), lipid profile, and stress oxidative are associated with susceptibility to psoriasis. One hundred patients with psoriasis and 100 sex- and age-matched unrelated healthy controls were recruited for this case-control study. ACE I/D and AT1R A1166 C polymorphisms were identified by the polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism, respectively, malondialdehyde (MDA) was detected by the high-performance liquid chromatography, serum arylesterase (ARE) activity of paraoxonase and catalase activities were detected by the spectrophotometry, superoxide dismutase (SOD) activity and vascular adhesion protein (VAP)-1 were measured by ELISA. The presence of C allele of AT1R A1166 C and I allele of ACE considerably increased the risk of psoriasis by 6.42-fold (P < 0.001). The distribution of II-genotype of ACE was significantly higher in psoriasis patients than in control group and increased the risk of disease by 3.11-times (P = 0.023). The higher levels of MDA in patients and the higher activity of SOD, ARE, and CAT was observed in healthy controls with I/D+I/I-genotype of ACE I/D. This study for the first time demonstrated that the ACE I/D and AT1R A 1166 C genes polymorphisms robustly increases the risk of developing psoriasis in population from west of Iran. In addition, these individuals had significantly higher VAP-1 and MDA concentration and lower enzymatic and nonenzymatic antioxidant-status, suggesting that psoriatic patients carrying C allele of AT1R1166 polymorphism may be more susceptible to cardiovascular disease and myocardial infarction compared with A allele.
Keyphrases
- angiotensin converting enzyme
- angiotensin ii
- end stage renal disease
- oxidative stress
- newly diagnosed
- cardiovascular disease
- chronic kidney disease
- ejection fraction
- prognostic factors
- high performance liquid chromatography
- atopic dermatitis
- staphylococcus aureus
- high resolution
- cell proliferation
- escherichia coli
- systemic lupus erythematosus
- coronary artery disease
- hydrogen peroxide
- patient reported outcomes
- dna damage
- induced apoptosis
- soft tissue
- atrial fibrillation
- ms ms
- patient reported
- molecular dynamics
- cystic fibrosis
- simultaneous determination
- solid phase extraction
- ankylosing spondylitis