Development and evaluation of a milk protein transcript depletion method for differential transcriptome analysis in mammary gland tissue.

Johanna BrodhagenRosemarie WeikardUlrike ThomAnnika HeimesJuliane GüntherFrieder HadlichHolm ZerbeWolfgang PetzlMarie M MeyerholzMartina HoedemakerHans-Joachim SchuberthSusanne EngelmannChrista Kuehn

Published in: BMC genomics (2019)

The implementation of selective RNase H-mediated RNA depletion of milk protein gene transcripts from the mammary gland transcriptome of lactating cows will be highly beneficial to establish comprehensive transcript catalogues of the tissue that better reflects its transcriptome complexity.

Keyphrases

rna seq
genome wide
single cell
gene expression
protein protein
healthcare
primary care
amino acid
binding protein
dna methylation
copy number
heat stress
nucleic acid