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Exploring antiproliferative activities and kinase profile of ortho-substituted N-(4-(2-(benzylamino)-2-oxoethyl)phenyl)benzamides.

Yosra A MuhammadAbdelsattar Mansour Omar EbeidFarid AhmedMaan T KhayatAzizah M MalebariSara M IbrahimShaza A MassMahmoud A ElfakyMoustafa E El-Araby
Published in: Chemical biology & drug design (2023)
Designing kinase inhibitors that bind to the substrate site of oncogenic kinases in a promising, albeit less explored, approach to kinase inhibition as it was sought to avoid the issue of untoward off-target modulations. Our previously identified compound KAC-12 with a meta-chlorophenyl substitution was an example of this approach. While it showed confirmed inhibitory activity against cancer cells, this substitution shifted the profile of affected targets away from Src/tubulin which were seen with the parent KX-01. In this paper, we synthesized compounds with ortho-substitutions, and we investigated the effect of such substitutions on their cellular and subcellular activities. The compound N-(4-(2-(benzylamino)-2-oxoethyl)phenyl)-2-(morpholine-4-carbonyl)benzamide (4) exhibited substantial activities against cell lines such HCT116 (IC 50 of 0.97 μM) and IC 50 HL60 (2.84 μM). Kinase profiling showed that compound 4 trended consistently with KAC-12 as it did not affect Src, but it had more impact on members of the Src family of kinases (SFK) such as Yes, Hck, Fyn, Lck, and Lyn. Both compounds exhibited profound downregulation effects on Erk1/2 but differed on others such as GSK3α/β and C-Jun. Collectively, this study further support to the hypothesis that small structural changes might bring higher changes in their kinome profile.
Keyphrases
  • tyrosine kinase
  • signaling pathway
  • protein kinase
  • cell proliferation
  • pi k akt
  • molecular docking
  • intellectual disability
  • transcription factor
  • mass spectrometry