Replicative transposition contributes to the evolution and dissemination of KPC-2-producing plasmid in Enterobacterales.
Yu TangGang LiPinghua ShenYing ZhangXiaofei JiangPublished in: Emerging microbes & infections (2021)
AbstractKlebsiella pneumoniae carbapenemase (KPC)-producing Enterobacterales are prevalent worldwide and pose an alarming threat to public health. The incidence and transmission of blaKPC-2 gene via horizontal gene transfer (e.g., transposition) have been well documented. However, the dynamics of transposon structure bearing blaKPC-2 and their exact effects on the evolution and dissemination of blaKPC-2 gene are not well characterized. Here, we collected all 161 carbapenem-resistant Enterobacterales (CRE) isolates during the early stage of CRE pandemic. We observed that the prevalence of KPC-2-producing Enterobacterales was mediated by multiple species and sequence types (STs), and that blaKPC-2 gene was located on three diverse variants of Tn1721 in multi-drug resistance (MDR) region of plasmid. Notably, outbreak of KPC-2-producing plasmid is correlated with the dynamics of transposon structure. Furthermore, we experimentally demonstrated that replicative transposition of Tn1721 and IS26 promotes horizontal transfer of blaKPC-2 and evolution of KPC-2-producing plasmid. The Tn1721 variants appearing concurrently with the peak of epidemic (A2- and B-type) showed higher transposition frequencies and certain superior ability to propagation. Overall, our work suggests replicative transposition contributes to the evolution and transmission of KPC-2-producing plasmid and highlights its important role in the inter- and intra-species dissemination of blaKPC-2 gene in Enterobacterales.
Keyphrases
- klebsiella pneumoniae
- escherichia coli
- multidrug resistant
- copy number
- public health
- genome wide
- early stage
- crispr cas
- genome wide identification
- drug resistant
- gram negative
- sars cov
- acinetobacter baumannii
- squamous cell carcinoma
- coronavirus disease
- risk factors
- radiation therapy
- genetic diversity
- gene expression
- pseudomonas aeruginosa