Assessment of Albumin ECM Accumulation and Inflammation as Novel In Vivo Diagnostic Targets for Multi-Target MR Imaging.
Jana MöckelJulia BrangschCarolin ReimannJan O KaufmannIngolf SackDilyana B MangarovaAvan KaderMatthias TaupitzLisa C AdamsSarah KellerAntje LudwigBernd HammRené M BotnarMarcus R MakowskiPublished in: Biology (2021)
Atherosclerosis is a progressive inflammatory vascular disease characterized by endothelial dysfunction and plaque burden. Extracellular matrix (ECM)-associated plasma proteins play an important role in disease development. Our magnetic resonance imaging (MRI) study investigates the feasibility of using two different molecular MRI probes for the simultaneous assessment of ECM-associated intraplaque albumin deposits caused by endothelial damage and progressive inflammation in atherosclerosis. Male apolipoprotein E-deficient (ApoE-/-)-mice were fed a high-fat diet (HFD) for 2 or 4 months. Another ApoE-/--group was treated with pravastatin and received a HFD for 4 months. T1- and T2*-weighted MRI was performed before and after albumin-specific MRI probe (gadofosveset) administration and a macrophage-specific contrast agent (ferumoxytol). Thereafter, laser ablation inductively coupled plasma mass spectrometry and histology were performed. With advancing atherosclerosis, albumin-based MRI signal enhancement and ferumoxytol-induced signal loss areas in T2*-weighted MRI increased. Significant correlations between contrast-to-noise-ratio (CNR) post-gadofosveset and albumin stain (R2 = 0.78, p < 0.05), and signal loss areas in T2*-weighted MRI with Perls' Prussian blue stain (R2 = 0.83, p < 0.05) were observed. No interference of ferumoxytol with gadofosveset enhancement was detectable. Pravastatin led to decreased inflammation and intraplaque albumin. Multi-target MRI combining ferumoxytol and gadofosveset is a promising method to improve diagnosis and treatment monitoring in atherosclerosis.
Keyphrases
- contrast enhanced
- magnetic resonance imaging
- high fat diet
- magnetic resonance
- diffusion weighted imaging
- computed tomography
- extracellular matrix
- oxidative stress
- mass spectrometry
- cardiovascular disease
- adipose tissue
- insulin resistance
- type diabetes
- air pollution
- cognitive decline
- metabolic syndrome
- mild cognitive impairment
- ms ms
- living cells
- photodynamic therapy
- high speed
- fluorescence imaging