Antioxidant and Cytoprotective Effects of New Diarylheptanoids from Rhynchanthus beesianus.
Pei-Feng ZhuGui-Guang ChengLan-Qin ZhaoAfsar KhanXing-Wei YangBu-Yun ZhangMeng-Cheng LiYa-Ping LiuXiao-Dong LuoPublished in: Journal of agricultural and food chemistry (2021)
Rhynchanthus beesianus (Zingiberaceae) has been an important food spice and vegetable in southern China. Fifteen phenolic compounds (1-15) including three new diarylheptanoids, rhynchanines A-C (1-3) and one new phenylpropanoid, 4-O-methylstroside B (9), were isolated from R. beesianus rhizomes. The structures of new compounds were elucidated by comprehensive analyses through NMR, HRMS technique, acid hydrolysis, and Mosher's reaction. Among them, compound 5 is the first isolated natural product and its NMR data are reported. Most of the isolated compounds, especially 3-6 and 8, showed significant antioxidant activities on DPPH, ABTS+ radical scavenging, and FRAP assays. Furthermore, the antioxidant phenolic compounds were evaluated for their cytoprotective capacity against H2O2-induced oxidative stress in HepG-2 cells. Compounds 3 and 5 could significantly inhibit reactive oxygen species production, and compounds 3, 5, and 6 could remarkably prevent the cell apoptosis. Then, the R. beesianus rhizome, which contained phenolic compounds, might serve as a functional food for potential application on preventing oxidative stress-connected diseases.
Keyphrases
- human health
- risk assessment
- oxidative stress
- high resolution
- reactive oxygen species
- magnetic resonance
- anti inflammatory
- dna damage
- cell proliferation
- diabetic rats
- machine learning
- high throughput
- solid state
- electronic health record
- induced apoptosis
- big data
- single cell
- high resolution mass spectrometry
- artificial intelligence
- data analysis