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Proteome allocation is linked to transcriptional regulation through a modularized transcriptome.

Arjun PatelDominic McGrossoYing HefnerAnaamika CampeauAnand V SastrySvetlana MauryaKevin RychelDavid J GonzalezBernhard O Palsson
Published in: Nature communications (2024)
It has proved challenging to quantitatively relate the proteome to the transcriptome on a per-gene basis. Recent advances in data analytics have enabled a biologically meaningful modularization of the bacterial transcriptome. We thus investigate whether matched datasets of transcriptomes and proteomes from bacteria under diverse conditions can be modularized in the same way to reveal novel relationships between their compositions. We find that; (1) the modules of the proteome and the transcriptome are comprised of a similar list of gene products, (2) the modules in the proteome often represent combinations of modules from the transcriptome, (3) known transcriptional and post-translational regulation is reflected in differences between two sets of modules, allowing for knowledge-mapping when interpreting module functions, and (4) through statistical modeling, absolute proteome allocation can be inferred from the transcriptome alone. Quantitative and knowledge-based relationships can thus be found at the genome-scale between the proteome and transcriptome in bacteria.
Keyphrases
  • genome wide
  • single cell
  • rna seq
  • gene expression
  • dna methylation
  • copy number
  • healthcare
  • high resolution
  • network analysis
  • artificial intelligence
  • electronic health record
  • high density