Ultrasensitive detection of pectin based on the decarboxylation reaction and surface-enhanced Raman spectroscopy.
Huan ZhangQi GuanPei ZengGuoqiang WuYanping HongWuying YangChunrong WangPublished in: Analytical methods : advancing methods and applications (2022)
In the present study, a novel simple and sensitive method was developed for the determination of pectin based on the decarboxylation derivatization reaction and surface-enhanced Raman scattering (SERS) without complicated separation steps. The derivatization reaction can be controlled by the experimental parameters such as reaction time, temperature and the amount of hydrochloric acid. Additionally, the method was used to accurately and reliably detect pectin added in honey or apple, which can be detected at levels as low as 0.5 or 0.01 ppm, respectively. Based on the conventional decarboxylation reaction, a simple and sensitive SERS method was proposed for the detection of pectin, which shows potential for practical application.
Keyphrases
- raman spectroscopy
- label free
- gold nanoparticles
- ms ms
- liquid chromatography
- solid phase extraction
- liquid chromatography tandem mass spectrometry
- sensitive detection
- gas chromatography mass spectrometry
- high performance liquid chromatography
- loop mediated isothermal amplification
- simultaneous determination
- tandem mass spectrometry
- mass spectrometry
- real time pcr
- climate change
- ultra high performance liquid chromatography
- high resolution mass spectrometry