3-Hydroxy-3-(2-oxopropyl)indolin-2-one, a product of a human-derived Enterocloster strain, is an inhibitor of nitric oxide production.
Shun SaitoTomoya BannoMidori A AraiPublished in: Bioscience, biotechnology, and biochemistry (2023)
When cultured anaerobically, Enterocloster sp. RD014215 was found to produce 1. Using nuclear magnetic resonance and mass spectroscopy, the planar structure of 1 was determined to be 3-hydroxy-3-(2-oxopropyl)indolin-2-one. The chirality of 1 was implied as S by comparing the optical rotation value of 1 with literature reports of the synthesized compounds. To our knowledge, this work represents the first discovery of the metabolite produced by Enterocloster strain. 1 exhibited inhibition of nitric oxide (NO) production, demonstrating a 50% inhibitory activity (IC50) of 34 μM for NO production by murine macrophage cells subjected to lipopolysaccharide (LPS) stimulation.
Keyphrases
- nitric oxide
- magnetic resonance
- endothelial cells
- inflammatory response
- high resolution
- induced apoptosis
- healthcare
- systematic review
- nitric oxide synthase
- small molecule
- hydrogen peroxide
- cell cycle arrest
- adipose tissue
- toll like receptor
- emergency department
- high throughput
- signaling pathway
- lps induced
- immune response
- induced pluripotent stem cells
- oxidative stress
- high speed
- anti inflammatory
- cell death
- adverse drug
- single cell
- electronic health record