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Aberration corrections of doughnut beam by adaptive optics in the turbid medium.

Chenxue WuJiajia ChenKe SiYanchun SongXinpei ZhuLejia HuYao ZhengWei Gong
Published in: Journal of biophotonics (2019)
The doughnut beam is a spatially structured beam which has been widely used in super-resolution microscopy, laser trapping and so on. However, when it passes through thick scattering medium, aberrations will seriously affect its performance. Currently, adaptive optics (AO) has become one of the most powerful tools to compensate aberrations. However, conventional AO always suffers from limited corrected field of view (FOV). Here, we propose a method with conjugate AO system based on coherent optical adaptive technique. The results show that the corrected FOV can be improved effectively. For a wide range of the optical applications with doughnut beam, our method has potentials in correcting aberrations with high speed in turbid media.(A) Mouse brain slice, (B) the distribution of r PAO , (C) the distribution of r CAO . The vortex beam focus of the blue point in (B) and (C) among a 137.5 × 137.5 μm FOV (D1) ideally, (D2) with scattering, (D3) in pupil AO system and (D4) in conjugate AO system.
Keyphrases
  • high speed
  • monte carlo
  • atomic force microscopy
  • high resolution
  • copy number
  • electron microscopy
  • cancer therapy
  • dna methylation
  • magnetic resonance
  • high throughput
  • single molecule
  • genome wide
  • mass spectrometry