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Quantitative Analysis and Optimization of Site-Specific Protein Bioconjugation in Mammalian Cells.

Amy RyanOlivia ShadeAnirban BardhanAleksander BartnikAlexander Deiters
Published in: Bioconjugate chemistry (2022)
Despite a range of covalent protein modifications, few techniques exist for quantification of protein bioconjugation in cells. Here, we describe a novel method for quantifying in cellulo protein bioconjugation through covalent bond formation with HaloTag. This approach utilizes unnatural amino acid (UAA) mutagenesis to selectively install a small and bioorthogonally reactive handle onto the surface of a protein. We utilized the fast kinetics and high selectivity of inverse electron-demand Diels-Alder cycloadditions to evaluate reactions of tetrazine phenylalanine (TetF) with strained trans -cyclooctene-chloroalkane (sTCO-CA) and trans -cyclooctene lysine (TCOK) with tetrazine-chloroalkane (Tet-CA). Following bioconjugation, the chloroalkane ligand is exposed for labeling by the HaloTag enzyme, allowing for straightforward quantification of bioconjugation via simple western blot analysis. We demonstrate the versatility of this tool for quickly and accurately determining the bioconjugation efficiency of different UAA/chloroalkane pairs and for different sites on different proteins of interest, including EGFP and the estrogen-related receptor ERRα.
Keyphrases
  • amino acid
  • protein protein
  • binding protein
  • oxidative stress
  • small molecule
  • crispr cas
  • south africa
  • signaling pathway
  • cell cycle arrest