A high solids field-to-fuel research pipeline to identify interactions between feedstocks and biofuel production.
Meenaa ChandrasekarLeela JoshiKarleigh KriegSarvada ChipkarEmily BurkeDerek J DebrauskeKurt D ThelenTrey K SatoRebecca Garlock OngPublished in: Biotechnology for biofuels (2021)
A pipeline of small-scale AFEX pretreatment and roller bottle enzymatic hydrolysis was able to provide adequate quantities of hydrolysate for respirometer fermentation experiments and was able to overcome hydrolysis bottlenecks at high solids loading by obtaining greater liquefaction compared to batch shake flask hydrolysis. Thus, the roller bottle method can be effectively utilized to compare divergent feedstocks and diverse process conditions.