Genome-scale CRISPR screening at high sensitivity with an empirically designed sgRNA library.

Luisa HenkelBenedikt RauscherBarbara SchmittJan WinterMichael Boutros

Published in: BMC biology (2020)

We show how empirically designed libraries in combination with an optimized experimental design increase the dynamic range in gene essentiality screens at reduced library coverage.

Keyphrases

genome wide
dna methylation
copy number
gene expression
transcription factor
single cell