Analytical validation of a novel targeted next-generation sequencing assay for mutation detection in thyroid nodule aspirates and tissue.

Antonella VerrientiValeria PecceLuana AbballeValeria RamundoRosa FalconeFarzaneh Inanloo Nigi JakChiara BrunelliGuido FaddaDaniela BoscoValeria AscoliRaffaella CarlettiCira Di GioiaGiorgio GraniMarialuisa Sponziello

Published in: Endocrine (2020)

This validation study of a novel NGS-based assay for thyroid nodules demonstrated that the assay can be reliably used on multiple thyroid sample types, including FNA from different sources and FF and FFPE thyroid samples, thus providing a robust and reliable assay to genotype thyroid nodules, which may improve thyroid cancer diagnosis and care.

Keyphrases

high throughput
healthcare
palliative care
copy number
gene expression
quality improvement
mass spectrometry