On the preservation of non-covalent protein complexes during electrospray ionization.
Konstantin ChinginKonstantin BarylyukHuanwen ChenPublished in: Philosophical transactions. Series A, Mathematical, physical, and engineering sciences (2017)
The application range of electrospray ionization mass spectrometry for the quantitative determination of stoichiometries and binding constants for non-covalent protein complexes is broadly discussed. The underlying fundamental question is whether or not the original molecular equilibrium can be preserved during the ionization process and be revealed by subsequent mass spectrometry analysis. Here, we take a new look at this question by discussing recent studies in droplet chemistry.This article is part of the themed issue 'Quantitative mass spectrometry'.
Keyphrases
- mass spectrometry
- gas chromatography
- high resolution
- liquid chromatography
- capillary electrophoresis
- high performance liquid chromatography
- binding protein
- protein protein
- solid phase extraction
- tandem mass spectrometry
- molecular dynamics
- high throughput
- single cell
- molecular dynamics simulations
- small molecule
- transcription factor
- molecularly imprinted
- simultaneous determination