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Evaluation of an isothermal amplification HPV detection assay for primary cervical cancer screening.

Wei ZhangHui DuXia HuangChun WangXianzhi DuanYan LiuBin ShiWei ZhangXinfeng QuLihui WeiMark SchiffmanJ L BelinsonRuifang Wu
Published in: Infectious agents and cancer (2020)
The AmpFire HPV assay showed similar sensitivity to Cobas and SeqHPV for CIN2+ and CIN3+ on both self and clinician-collections (P>0.05), with good specificity. The speed, low cost, and simplicity of this assay will make it particularly suited for low and middle resource settings. Its accuracy with self-collection makes it applicable for mass screening programs.
Keyphrases
  • cervical cancer screening
  • low cost
  • high throughput
  • high grade
  • nucleic acid
  • public health
  • label free
  • loop mediated isothermal amplification
  • real time pcr