Mangiferin potentiates the activity of antifungal agents against fluconazole-resistant Candida spp.
Thais Lima FerreiraAmanda Cavalcante LeitãoLisandra Juvêncio da SilvaLívia Gurgel do Amaral Valente SáVitória Pessoa de Farias CabralDaniel Sampaio RodriguesSarah Alves BarbosaJoão Batista de Andrade NetoAmanda Dias BarbosaLara Elloyse Almeida MoreiraMaria Erivanda França RiosBruno Coêlho CavalcantiManoel Odorico de MoraesHélio Vitoriano Nobre JúniorCecília Rocha da SilvaPublished in: Future microbiology (2024)
Aim: To evaluate the antifungal activity of mangiferin against Candida spp. resistant to fluconazole. Materials & methods: The antifungal activity of mangiferin was assessed using broth microdilution and its interaction with azoles and amphotericin B was evaluated by checkerboard. The activity of mangiferin against Candida spp. biofilms was assessed using the MTT colorimetric assay and its possible mechanism of action was evaluated using flow cytometry. Results: Mangiferin showed activity against Candida albicans, Candida tropicalis and Candida parapsilosis resistant to fluconazole and showed synergism with azoles and amphotericin B. Mangiferin increased the activity of antifungals against Candida biofilms and caused depolarization of the mitochondrial membrane and externalization of phosphatidylserine, suggesting apoptosis. Conclusion: mangiferin combined with antifungals has potential against Candida spp.