A validated eco-friendly HPLC-FLD for analysis of the first approved antiviral remdesivir with other potential add-on therapies for COVID-19 in human plasma and pharmaceuticals.
Asmaa M TahaWafaa S HassanManal S ElmasryRania Adel SayedPublished in: Analytical methods : advancing methods and applications (2023)
It is crucial to have a reliable and sensitive method for separating common drugs used in SARS-CoV-2 pneumonia treatment protocols for ongoing treatment and upcoming investigations. This study presents an HPLC-FLD approach to analyze three co-administered medicines - remdesivir (RDV), hydroxychloroquine sulphate (HCQ), and levofloxacin hemihydrate (LVX) - in their pure forms, pharmaceutical preparations, and spiked human plasma. The HPLC-FLD analysis was conducted using a Symmetry® C18 column (100 mm × 4.6 mm ID, 3.5 μm particle size) at 40 °C, with (A) an aqueous mixture of 0.02 M phosphate buffer and 0.2% heptane-1-sulphonic acid sodium solutions (50 : 50) adjusted to pH 3, (B) acetonitrile, and (C) methanol as the mobile phase. The injection volume was 10 μL, and the flow rate was 1.5 mL min -1 . The detection was done using a multi-wavelength excitation and emission fluorescence detector, with individual optimization for each drug. The drug separation time was less than 10 minutes, and the method showed sensitive and wide linearity ranges for all medicines, with r 2 values of more than 0.999. The impact of the mobile phase pH and flow rate on suitability parameters (retention time and number of theoretical plates) was studied. The method was found to be environmentally friendly based on GAPI and AGREE metrics. The validity of the method was evaluated following ICH and FDA guidelines.
Keyphrases
- sars cov
- ms ms
- simultaneous determination
- solid phase extraction
- mass spectrometry
- high performance liquid chromatography
- coronavirus disease
- liquid chromatography
- magnetic resonance imaging
- emergency department
- computed tomography
- climate change
- intensive care unit
- respiratory syndrome coronavirus
- carbon dioxide
- ultrasound guided
- high resolution
- low cost
- acute respiratory distress syndrome
- energy transfer
- mechanical ventilation
- sensitive detection