Microbiological profile and infection potential of different cryopreserved skull flaps after decompressive hemicraniectomy. Is cryopreservation at - 80 ℃ better?
Rachit AgrawalC RompfA B PranadaP VollmarA De LorenzoA HoyerK GousiasPublished in: BMC research notes (2022)
A total of 17 cryopreserved skull flaps (8: - 23 °C; 9: - 80 °C) explanted during decompressive hemicraniectomies performed between 2019 and 2020 as well as 2 computer-aided-designed skulls (1 vancomycin-soaked) were analyzed. Median duration of cryopreservation was 10.5 months (2-17 months). No microorganisms were detected at the normal bacterial culture. After active contamination of our skull flaps, all samples showed similar bacterial growth of above-mentioned pathogens; thus, our study did not reveal an influence of the storage temperature upon infectious dynamic of the skulls.
Keyphrases
- soft tissue
- breast reconstruction
- umbilical cord
- risk assessment
- severe traumatic brain injury
- human health
- cord blood
- middle cerebral artery
- drinking water
- genome wide
- mesenchymal stem cells
- single cell
- methicillin resistant staphylococcus aureus
- gram negative
- gene expression
- climate change
- antimicrobial resistance
- dna methylation
- heavy metals