Whole genome sequencing for drug resistance determination in Mycobacterium tuberculosis.
Shaheed Vally OmarLavania JosephHalima M SaidFarzana IsmailNabila IsmailThabisile L GwalaNazir Ahmed IsmailPublished in: African journal of laboratory medicine (2019)
South Africa remains challenged with a high tuberculosis burden accompanied by an increase in drug resistant cases. We assessed the use of the Illumina MiSeq, a next-generation sequencing platform for whole genome sequencing, followed by bioinformatic analysis using a commercial software package to determine resistance to selected drugs used for Mycobacterium tuberculosis treatment in our setting. Whole genome sequencing shows potential as a diagnostic platform for the detection of drug resistance in Mycobacterium tuberculosis with the provision of information for several drugs simultaneously.
Keyphrases
- mycobacterium tuberculosis
- drug resistant
- south africa
- multidrug resistant
- pulmonary tuberculosis
- acinetobacter baumannii
- high throughput
- palliative care
- hiv positive
- risk factors
- emergency department
- copy number
- health information
- human health
- circulating tumor
- label free
- drug induced
- loop mediated isothermal amplification
- social media
- solid phase extraction
- quantum dots
- high throughput sequencing