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Links between fecal microbiota and the response to vaccination against influenza A virus in pigs.

Marion BoreyFany BlancGaëtan LemonnierJean-Jacques LeplatDeborah JardetMarie-Noëlle RossignolLaure RavonYvon BillonMaria BernardJordi EstelléClaire Rogel-Gaillard
Published in: NPJ vaccines (2021)
This study describes the associations between fecal microbiota and vaccine response variability in pigs, using 98 piglets vaccinated against the influenza A virus at 28 days of age (D28) with a booster at D49. Immune response to the vaccine is measured at D49, D56, D63, and D146 by serum levels of IAV-specific IgG and assays of hemagglutination inhibition (HAI). Analysis of the pre-vaccination microbiota characterized by 16S rRNA gene sequencing of fecal DNA reveals a higher vaccine response in piglets with a richer microbiota, and shows that 23 operational taxonomic units (OTUs) are differentially abundant between high and low IAV-specific IgG producers at D63. A stronger immune response is linked with OTUs assigned to the genus Prevotella and family Muribaculaceae, and a weaker response is linked with OTUs assigned to the genera Helicobacter and Escherichia-Shigella. A set of 81 OTUs accurately predicts IAV-specific IgG and HAI titer levels at all time points, highlighting early and late associations between pre-vaccination fecal microbiota composition and immune response to the vaccine.
Keyphrases
  • immune response
  • gene expression
  • single molecule
  • genome wide
  • dna methylation
  • circulating tumor
  • inflammatory response
  • transcription factor