Effects of Amiloride, an Ion Channel Blocker, on Alamethicin Pore Formation in Negatively Charged, Gold-Supported, Phospholipid Bilayers: A Molecular View.
Fatemeh AbbasiZhangFei SuJulia Alvarez-MalmagroJ Jay LeitchJacek LipkowskiPublished in: Langmuir : the ACS journal of surfaces and colloids (2019)
The effects of amiloride on the structure and conductivity of alamethicin ion pore formation within negatively charged, gold-supported, 1,2-dimyristoyl- sn-glycero-3-phosphocholine/Egg-PG membranes were investigated with the help of electrochemical impedance spectroscopy (EIS), photon polarization modulation-infrared reflection spectroscopy (PM-IRRAS), and atomic force microscopy (AFM). The EIS results indicate that ion conductivity across negatively charged phospholipid bilayers containing alamethicin decreases by an order of magnitude when amiloride is introduced to the system. Despite the reduction in ion conductivity, the PM-IRRAS data shows that amiloride does not inhibit ion channel formation by alamethicin peptides. High-resolution AFM images revealed that amiloride enlarges and distorts the shape of alamethicin ion pores when introduced to the system, indicating that it is inserting itself into the mouth of the alamethicin pores. This effect is driven by electrostatic interactions between positively charged amiloride molecules and the negative charge on the membrane.
Keyphrases
- atomic force microscopy
- single molecule
- high resolution
- high speed
- molecular dynamics simulations
- particulate matter
- air pollution
- living cells
- heavy metals
- deep learning
- gold nanoparticles
- machine learning
- magnetic resonance imaging
- big data
- silver nanoparticles
- ionic liquid
- simultaneous determination
- contrast enhanced