Detection of Pathogens and Antimicrobial Resistance Genes in Ventilator-Associated Pneumonia by Metagenomic Next-Generation Sequencing Approach.

Ting ChenLei ZhangWenhua HuangHuijun ZongQian LiYuling ZhengQingyu LvDecong KongYuhao RenYongqiang JiangYan LiPeng Liu

Published in: Infection and drug resistance (2023)

In this study, we developed a workflow that can accurately detect VAP pathogens and enable prediction of antimicrobial resistance phenotypes within 5 h of sample receipt by mNGS.

Keyphrases

antimicrobial resistance
genome wide
copy number
loop mediated isothermal amplification
label free
gene expression
intensive care unit
real time pcr
microbial community
gram negative
respiratory failure
antibiotic resistance genes