Discovery, absolute assignments, and total synthesis of asperversiamides A-C and their potent activity against Mycobacterium marinum.

Integrating molecular networking and 1H NMR techniques successfully enabled the discovery of three new cycloheptapeptides, asperversiamides A-C (1-3). Their complete structures were further confirmed by total synthesis. All three compounds exhibited potent inhibitory activity against Mycobacterium marinum.