Tuning Bacterial Morphology to Enhance Anticancer Vaccination.
Chu-Xin LiYongdan QiYingge ChenYu ZhangBin LiJun FengXian-Zheng ZhangPublished in: ACS nano (2023)
Morphology tuning is a potent strategy to modulate physiological effects of synthetic biomaterials, but it is rarely explored in microbe-based biochemicals due to the lack of artificial adjustability. Inspired by the interesting phenomenon of microbial transformation, Escherichia coli is rationally adjusted into filamentous morphology-adjusted bacteria (MABac) via chemical stimulation to prepare a bacteria-based vaccine adjuvant/carrier. Inactivated MABac display stronger immunogenicity and special delivery patterns (phagosome escape and cytoplasmic retention) that are sharply distinct from the short rod-shaped bacteria parent (Bac). Transcriptomic study further offers solid evidence for deeply understanding the in vivo activity of MABac-based vaccine, which more effectively motivates multiple cytosolic immune pathways (such as NOD-like receptors and STING) and induces pleiotropic immune responses in comparison with Bac. Harnessing the special functions caused by morphology tuning, the MABac-based adjuvant/carrier significantly improves the immunogenicity and delivery profile of cancer antigens in vivo, thus boosting cancer-specific immunity against the melanoma challenge. This study validates the feasibility of tuning bacterial morphology to improve their biological effects, establishing a facile engineering strategy that upgrades bacterial properties and functions without complex procedures like gene editing.
Keyphrases
- escherichia coli
- papillary thyroid
- immune response
- early stage
- squamous cell carcinoma
- dendritic cells
- young adults
- staphylococcus aureus
- single cell
- microbial community
- multidrug resistant
- artificial intelligence
- toll like receptor
- reduced graphene oxide
- cystic fibrosis
- deep learning
- gold nanoparticles
- lymph node metastasis
- metal organic framework