Multiplexed microRNA Detection Using Metal-Organic Framework for Signal Output.

A specificity fluorescence platform for probing of sequence-specific miRNA using fluorophore labeled DNA and metal-organic frameworks (MOFs) is developed. Ni-IRMOF-74-II can quench the fluorescence of four fluorescent dyes labeled in the single strand oligodeoxynucleotides (ssODNs), and fluorescence recovers in the presence of their target miRNA and can be detected in their corresponding channels. Furthermore, recovered fluorescence intensity has a linear relationship with the concentration of the target miRNA in the range from 1.25 to 100 nM at the four corresponding channels. This fluorescence sensor platform can detect several kinds of miRNA in the same system and at same time with the high specificity and negligible cross-reactivity.