Glioma 2021 WHO Classification: The Superiority of NGS Over IHC in Routine Diagnostics.

Paulina Śledzińska Marek G Bebyn Ewelina Szczerba Jacek Furtak Mateusz Szylberg Natalia Olszewska Katarzyna Kamińska Janusz Kowalewski Marzena Anna Lewandowska

Published in: Molecular diagnosis & therapy (2022)

We demonstrated that qPCR or NGS performed in reference genetic laboratories, rather than IHC, is the most reliable method for IDH1/2 analysis. Clinicians should be aware of discrepancies in MLPA or IHC results and seek reconsultation in facilities with extensive access to advanced molecular technologies. Moreover, we proposed a new algorithm for the molecular diagnostic procedures in glioma patients based on the WHO CNS5.

Keyphrases

end stage renal disease
machine learning
deep learning
ejection fraction
newly diagnosed
chronic kidney disease
peritoneal dialysis
prognostic factors
palliative care
genome wide
blood brain barrier
low grade
gene expression
dna methylation
copy number
data analysis