Evaluation of tenofovir disoproxil fumarate loaded silver nanoparticle on testicular morphology in experimental type-2 diabetic rats.
Samuel Oluwaseun OlojedeSodiq Kolawole LawalAyobami DareEdwin Coleridge Stephen NaiduCarmen Olivia RennieOnyemaechi Okpara AzuPublished in: Artificial cells, nanomedicine, and biotechnology (2022)
Reproductive derangement and metabolic disorders in human immunodeficiency virus (HIV) infected persons require a nanoparticle delivery system to convey antiretroviral drugs to the anatomical sanctuary such as testis. This study investigated the effects of tenofovir disoproxil fumarate (TDF) loaded silver nanoparticles (AgNPs) on the testicular oxidative stress, inflammatory cytokines and histology in male diabetic rats. Thirty-six Sprague-Dawley rats weighing 230 ± 20 g were randomly divided into diabetic and non-diabetic groups ( n = 18). Diabetes was induced using the fructose-streptozotocin (Frt-STZ) rat model. Both groups were further divided into three ( n = 6) and administered distilled water, TDF, or TDF-AgNP. Results obtained with the TDF-AgNP administration showed a significant increase ( p < .05) in the reduced glutathione and catalase levels. Tumour necrosis factor-alpha and interleukin 6 were reduced in diabetic rats administered TDF-AgNP. More so, administration of TDF-AgNP to diabetic rats improved testicular histoarchitecture in diabetic rats. In addition, diabetic rats administered TDF-AgNP showed a significant reduction ( p < .05) in blood glucose levels. TDF-AgNP to diabetic rats enhanced testicular antioxidant enzyme, reduced testicular inflammation, and alleviated structural derangements in the testis. Thus, the application of AgNP to deliver TDF may alleviate testicular toxicity and subsequently cater for neglected reproductive dysfunction during the management of HIV infection.
Keyphrases
- diabetic rats
- oxidative stress
- germ cell
- human immunodeficiency virus
- antiretroviral therapy
- hiv infected
- silver nanoparticles
- blood glucose
- induced apoptosis
- dna damage
- type diabetes
- ischemia reperfusion injury
- hiv aids
- hiv positive
- hepatitis c virus
- hiv infected patients
- drug delivery
- cardiovascular disease
- wound healing
- glycemic control
- mass spectrometry
- high resolution
- insulin resistance
- heat shock
- skeletal muscle
- adipose tissue
- weight loss
- drug induced
- heat stress