Redesigning N-glycosylation sites in a GH3 β-xylosidase improves the enzymatic efficiency.
Marcelo Ventura RubioCésar Rafael Fanchini TerrasanFabiano Jares ContesiniMariane Paludetti ZubietaJaqueline Aline GerhardtLeandro Cristante OliveiraAny Elisa de Souza Schmidt GonçalvesFausto AlmeidaBradley Joseph SmithGustavo Henrique Martins Ferreira de SouzaArtur Hermano Sampaio DiasMunir SkafAndré Ricardo de Lima DamasioPublished in: Biotechnology for biofuels (2019)
This study demonstrates the influence of N-glycosylation on A. nidulans BxlB production and function, reinforcing that protein glycoengineering is a promising tool for enhancing thermal stability, secretion, and enzymatic activity. Our report may also support biotechnological applications for N-glycosylation modification of other CAZymes.