Glucose withdrawal induces Endothelin 1 release with significant angiogenic effect from first trimester (FTM), but not term human umbilical cord perivascular cells (HUCPVC).
Peter SzarazPoonam ManderNadav GasnerMax LibrachFarwah IqbalClifford LibrachPublished in: Angiogenesis (2019)
Low-glucose conditions had a significant post-transcriptional inductive effect on FTM HUCPVC angiogenic factor secretion, resulting in significantly higher VEGFc and Endothelin 1 release in 3 days compared to term counterparts. Conditioned media from low-glucose FTM HUCPVC cultures had a significantly higher endothelial network enhancing effect compared to all other experimental groups both in vitro aortic ring assay and in subcutan Matrigel™ plugs. Endothelin 1 depletion of the low-glucose FTM HUCPVC conditioned media significantly diminished its angiogenic effect CONCLUSIONS: FTM HUCPVC isolated from an early extraembryonic tissue show significant pro-angiogenic paracrine reaction in low-glucose conditions at least in part through the excess release of Endothelin 1. This can be a substantial advantage in cell therapy applications for ischemic injuries.
Keyphrases
- cell therapy
- mesenchymal stem cells
- blood glucose
- endothelial cells
- umbilical cord
- preterm infants
- gene expression
- bone marrow
- heart failure
- transcription factor
- type diabetes
- blood pressure
- oxidative stress
- high throughput
- cell proliferation
- skeletal muscle
- ischemia reperfusion injury
- single cell
- pulmonary arterial hypertension